Optimization of oligonucleotides for 5'- and 3'-rapid amplification of cDNA ends (race) comprises checking of compatibility on amplification by PCR

摘要

The optimization of oligonucleotides for RACE consists of checking of compatibility of the 5'- and 3'-RACE oligonucleotides on amplification by PCR. The RACE experiment clones a gene of interest/fragment of cDNA.