A rapid micro-PCR system for hepatitis C virus amplification

机译：用于丙型肝炎病毒扩增的快速微PCR系统

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摘要

A rapid micro-polymerase chain reaction (mu-PCR) system was integrated to amplify the complementary DNA (cDNA) moleules of hepatitis C virus (HCV). This system consits of a rapid thermal cycling system and a muPCR chip fabricated by MEMS fabrication techniques. This rapid muPCR system is verified by using serum samples from patients with chronic hepatitis C. The HCV amplicon of the rapid muPCR system was analyzed by slab gel electrophoresis with spearation of DNA marker in parallel.

机译：集成了快速微聚合酶链反应（mu-PCR）系统，以扩增丙型肝炎病毒（HCV）的互补DNA（cDNA）分子。该系统由快速热循环系统和通过MEMS制造技术制造的muPCR芯片组成。通过使用慢性丙型肝炎患者的血清样品验证了该快速muPCR系统。通过平板凝胶电泳与DNA标记物的平行电泳，对快速muPCR系统的HCV扩增子进行了分析。

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来源
《Conference on micromachined devices and components》|2000年|p.188-192|共5页
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作者
Yu-Cheng Lin; Ming-Yuan Huang; Kung-Chia Young; Ting-Tsung Chang; Ching-Yi Wu;
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中图分类无线电电子学、电信技术;
关键词
KNA; PCR chip; MEMS; HCV;

机译：KNA; PCR芯片; MEMS;丙型肝炎病毒;

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1. Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method [J] . Lianlian Duan, Yefu Wang, Shawn S Li, BMC Infectious Diseases . 2005,第1期

机译：基于纳米金免疫扩增和银染法的蛋白质芯片测定法，可同时快速检测人乙型肝炎病毒和丙型肝炎病毒抗体
2. Detection of infections with hepatitis B virus, hepatitis C virus, and human immunodeficiency virus by analyses of dried blood spots - performance characteristics of the ARCHITECT system and two commercial assays for nucleic acid amplification [J] . R S Ross, Oumaima Stambouli, Nico Grüner, Virology Journal . 2013,第1期

机译：通过分析干血斑来检测乙型肝炎病毒，丙型肝炎病毒和人类免疫缺陷病毒的感染-ARCHITECT系统的性能特点和两种用于核酸扩增的商业检测
3. Detection of Hepatitis B Virus Using Micro-PCR and Real-Time PCR Methods [J] . Won Kang, Sang-Bum Park, Youn-Hyoung Nam Journal of the Korean Chemical Society . 2007,第1期

机译：使用Micro-PCR和实时PCR方法检测乙型肝炎病毒
4. A rapid micro-PCR system for hepatitis C virus amplification [C] . Yu-Cheng Lin, Ming-Yuan Huang, Kung-Chia Young, Conference on micromachined devices and components . 2000

机译：一种用于丙型肝炎病毒扩增的快速微型PCR系统
5. A serosurvey of human immunodeficiency virus, hepatitis B virus, and hepatitis C virus infection among inmates entering the California correctional system. [D] . Ruiz, Juan Domingo. 1997

机译：对进入加利福尼亚教养系统的囚犯进行的人类免疫缺陷病毒，乙型肝炎病毒和丙型肝炎病毒感染的血清调查。
6. Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method [O] . Lianlian Duan, Yefu Wang, Shawn Shun-cheng Li, 2005

机译：基于纳米金免疫扩增和银染法的蛋白质芯片测定法可同时快速检测人乙型肝炎病毒和丙型肝炎病毒抗体
7. Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method [O] . Wan Zhixiang, Li Shawn, Wang Yefu, 2005

机译：基于纳米金免疫扩增和银染法的蛋白质芯片测定法，可同时快速检测人乙型肝炎病毒和丙型肝炎病毒抗体

A rapid micro-PCR system for hepatitis C virus amplification

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