Biochemical and Physiological Mechanisms Related to Vernalization, Atonik and Benzyl Adenine in Pisum sativum

摘要

Vernalization at 5deg C for 5 days, either singly or in combination with a foliar application of atonik at 250, 500 and 1000 mg/1 or 6-benzyl adenine (BA) at 25, 50 and 100 mg/1 was studied on the growth parameters and flowering response, photosynthetic pigments, different carbohydrate and nitrogen fractions, ion contents and endogenous level of different phytohormones of Pisum sativum (cv. 'Master Bean'). All determined growth parameters (root and shoot length, fresh weight and dry weight; number ofnodes/plant; number of leaves/plant; total leaf area/plant; relative water content; number of flowers/plant) decreased in response to vernalization treatment. In contrast, vernalization in combination with 1000 mg/1 atonik or 50 mg/1 BAled to a significant increase in these parameters. Vernalization, alone or in combination with atonik or BA, significantly increased all photosynthetic pigments and generally led to a significant increase in different carbohydrate and nitrogen fractions and ion content.On the other hand, vernalization led to a significant decrease in total auxins, gibberellic acid (GA3) and different CK fractions (zeatin, kinetin and BA) in pea plant shoots; ABA increased significantly. In confrast, vemalization combined with atonik orBA at any concentration led to a progressive increase in total auxins, GA3 and different CK fractions while ABA decreased significantly compared with confrol values.