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Antioxidative Activities and Phenolic Content of Extracts from Okra (Abelmoschus esculentus L.)

机译:黄秋葵(Abelmoschus esculentus L.)提取物的抗氧化活性和酚类含量

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The aim of this study was to determine the antioxidant activities, TPC and to identify phenolic compounds of seeds and pulped okra (Abelmoschus esculentus L.), a vegetable that is consumed worldwide because it plays an important role in the human diet by supplying carbohydrates, minerals and vitamins. The antioxidant activities of seeds and pulped okra were evaluated by DPPH radical scavenging and ABTS radical cation decolorization assays were used. Total Phenolic Content (TPC) was determined using the folin-ciocalteu assay. I dentification of phenolic compounds was achieved using HPLC with the UV-diode array detection. The TPC of pulped and seeds of okra extracts were 10.75±0.02 mg GAE/100 g e xtract and 142.48±0.02 mg GAE/100 g extract which corresponding with scavenging activities. The predominant phenolic compound was procycanidin B2, following procycanidin B1 and rutin in seeds but in pulped okra they were identified as catechin, procycanidin B2, epicatechin and rutin. These findings in associations with the high content of antioxidant activity and phenolics in okra enhance the importance of this food stuff for the human diet.
机译:这项研究的目的是确定抗氧化剂活性,TPC并确定种子和果黄秋葵(Abelmoschus esculentus L.)的酚类化合物。黄秋葵是世界范围内广泛食用的蔬菜,因为它通过供应碳水化合物在人类饮食中起着重要的作用,矿物质和维生素。通过DPPH自由基清除法评估种子和黄秋葵的抗氧化活性,并使用ABTS自由基阳离子脱色测定法。总酚含量(TPC)使用folin-ciocalteu分析法测定。使用HPLC和UV二极管阵列检测仪可以鉴定酚类化合物。黄秋葵提取物的果肉和种子的TPC分别为10.75±0.02 mg GAE / 100 g提取物和142.48±0.02 mg GAE / 100 g提取物,具有清除活性。酚类化合物中的主要酚类成分是原花青素B2,继种子中的原花青素B1和芦丁之后,但在黄秋葵浆中,它们被鉴定为儿茶素,原花青素B2,表儿茶素和芦丁。这些发现与黄秋葵中高含量的抗氧化剂和酚类有关,增强了这种食品对人类饮食的重要性。

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