せん断応力負荷下で内皮細胞を培養した疑似血管マイクロデバイスの開発と形態変化の計測

摘要

微小血管系は細動脈・毛細血管・細静脈などの微小血管rn約三細リンパ管などのリンパ系および細胞組織間で構成rnされており，ここを封しる血液は生体の内田環境を恒常的rnに維持するために不可欠な酸素や養分を組織細胞に供給しrnている．%In order to investigate vascular diseases such as cause of atherosclerosis and myocardial infarction, relationships of endothelial cells (ECs) covered with surface blood vessels and blood flow stimulation have been experimentally studied. In the study, a blood vessel model for in vitro experiment made from polydimethylsiloxane (PDMS) microchip with a straight microchannel with 400 μm width and 75 μm depth was developed. By optimizing cells cultured condition such as the liquid introduction method and the surface coating for enhancement of cell attachment on the microchannel wall, a cell culture method in the microchannel with fluid shear stress to be confluent were developed. The morphological response, such as shape index, area and orientation, of cultured ECs with shear stress 0.1 Pa was investigated. In the period of low cell density until 60 hours culture, cells were elongated and aligned with flow direction. In the period of over about 50% cell density of confluent, cells became gradually randomly distributed. The area of cell at confluent with shear stress became 1.7 times larger than that of cell without shear stress. The results indicated that interaction of surrounding cells influenced the morphological response of ECs.