Selective colorimetric detection of dissolved ammonia in water via modified Berthelot's reaction on porous paper

摘要

Highlights•New ammonia determination method for water samples using the separation of ammonia gas followed by the Berthelot’s reaction.•Involvement of water-assisted mechanism in the selective indophenol formation reaction between a solid sensing layer and gaseous ammonia.•Fabrication of solid-state colorimetric ammonia sensors on a paper substrate.AbstractContinuous monitoring and rapid on-site determination of toxic ammonia in various aqueous systems are important for maintaining water quality and preserving the environment. Though several methods such as flow-based spectrophotometry are currently utilized for this purpose, there is still need for more selective ammonia determination. We present a new detection route for quantifying NH3in water samples by means of the evaporation of dissolved ammonia into headspace, followed by selective detection using a colorimetric sensor. Gaseous NH3detection is attained by the selective formation of blue indophenol dye through modified Berthelot’s reaction on porous paper. To optimize the sensing layer in terms of uniform and intense coloration, the water-soluble powder reagents were homogeneously dispersed in nonpolar cyclohexane and then deposited by a vacuum-assisted filtration on the paper substrate. The formation of indophenol blue was activated under highly humidified conditions through selective chemical reactions between solid-state Berthelot’s reagents and NH3. The colorimetric response of the low-cost paper sensors was saturated within 10min, and color change was observed by the naked eye for an ammonia solution of 10mg/L. Furthermore, the response magnitude was not affected by addition of 0.1wt% polar organic solvents (ethanol, acetone, dimethylformamide or acetic acid) or inorganic salts (NaCl, KCl, MgSO4or CaSO4), suggesting a good detection cross-selectivity.