SCREENING HIGH-AFFINITY MONOCLONAL ANTIBODIES

摘要

Terry Pearson, Professor of Biochemistry and Microbiology, University of Victoria, Canada, and CSO, SISCAPA Assay Technologies, Inc., a company that uses anti-peptide antibodies and mass spectrome-try to measure biomarkersrnIdentify monoclonal antibodies with high binding affinities for peptides present at low concentrations in biological mixtures.rnMany researchers use peptides as antibody-binding substrates, but this approach is fraught with complications. The Y-shaped antibody can bind two peptides simultaneously, which can artificially increase affinity measurements by several hundredfold, Pearson says.