Amplification, analysis and chromosome mapping of novel homeobox-containing and homoebox-flanking sequences in rice

摘要

Homeobox genes, widely distributed among animal and plant kingdoms, play an important role in devel- opmental process. several homeobox conserved fragments were amplified by PCR and the flanking regions were also obtained by an LM-PCR procedure. Sequencing and Southern analysis showed that they belong to a homebox gene family of rice. Six homeobox-containing fragments were mapped on the molecular linkage map of rice. They were lo- cated on chromosomes 3,4 and 7 respectively. It is noteworthy that there are 4 homebox fragments located on rice chromosome 3 and the result is also consistent with the comparative genomics between rice and maize.