DNA SEQUENCES DEDUCED FROM CHROMOSOMAL DNA OF PROPANE-OXIDIZING BACTERIA, COMPRISING prmA OR prmD GENE, OLIGONUCLEOTIDES COMPLEMENTARY TO SAID SEQUENCES, METHODS FOR THE IDENTIFICATION AND QUANTITATIVE DETERMINATION OF SAID BACTERIA, A KIT FOR THE IDENTIFICATION OF THE PRESENCE OF SAID BACTERIA, USE OF SEQUENCES OF prmA AND prmD GENES FOR THE IDENTIFICATION OF PRIMERS FOR GENE AMPLIFICATION AND A METHOD FOR DISCOVERING THE PRESENCE OF OIL OR NATURAL GAS RESERVOIRS

摘要

The invention relates to a method for the identification of propane-oxidizing bacteria which is based on the identification of at least one fragment of the prmA gene encoding the alpha subunit of the propane monooxygenase enzyme and/or the prmD gene encoding an ancillary protein involved in the oxidation reaction of propane by gene amplification in the presence of pairs of primers selected in correspondence of homologous portions, deduced from the alignment of the prmA and prmD sequences.