...
首页> 外文期刊>Nuclear Instruments & Methods in Physics Research. Section A, Accelerators, Spectrometers, Detectors and Associated Equipment >Photon counting imaging and centroiding with an electron-bombarded CCD using single molecule localisation software
【24h】

Photon counting imaging and centroiding with an electron-bombarded CCD using single molecule localisation software

机译:使用单分子定位软件的电子轰击CCD进行光子计数成像和质心

获取原文
获取原文并翻译 | 示例
           

摘要

Photon event centroiding in photon counting imaging and single-molecule localisation in super-resolution fluorescence microscopy share many traits. Although photon event centroiding has traditionally been performed with simple single-iteration algorithms, we recently reported that iterative fitting algorithms originally developed for single-molecule localisation fluorescence microscopy work very well when applied to centroiding photon events imaged with an MCP-intensified CMOS camera. Here, we have applied these algorithms for centroiding of photon events from an electron-bombarded CCD (EBCCD). We find that centroiding algorithms based on iterative fitting of the photon events yield excellent results and allow fitting of overlapping photon events, a feature not reported before and an important aspect to facilitate an increased count rate and shorter acquisition times.
机译:光子计数成像中的光子事件质心和超分辨率荧光显微镜中的单分子定位具有许多特征。尽管光子事件质心传统上是通过简单的单迭代算法执行的,但我们最近报道说,最初为单分子定位荧光显微镜开发的迭代拟合算法在将质子事件用于MCP增强CMOS相机成像的质心事件时效果很好。在这里,我们将这些算法应用于来自电子轰击CCD(EBCCD)的光子事件的质心。我们发现,基于光子事件的迭代拟合的质心算法可产生出色的结果,并且可以拟合重叠的光子事件,这是以前未曾报道过的功能,也是促进增加计数率和缩短采集时间的重要方面。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号