Neuroprotection of GST, an extract of traditional Chinese herb, against ischemic brain injury induced by transient brain ischemia and reperfusion in rat hippocampus

摘要

In this study, we investigated the effect of GST, an extract of Chinese traditional herb, onntransient brain ischemia/reperfusion-induced neuronal cell death. Immunoblotting was used tondetect the phosphorylation of MLK, JNK and c-jun. Transient (15 minutes) brain ischemia wasninduced by the four-vessel occlusion in Sprague–Dawley rats. GST was administrated to the SDnrats 20 minutes before ischemia or 1 hour after ischemia. Our data showed that the pretreatmentnof GST could inhibit phosphorylation of MLK, JNK and c-jun. Moreover, GST showednpotent neuroprotective effects on ischemic brain damage in vivo and administration of it 1 hournafter ischemia also achieved the protective effects. These results indicate that GST has anprominent neuroprotection action against brain ischemic damage and provides a promisingntherapeutic approach for ischemic brain injury. [Neurol Res 2008; 30: 471–475