MicroRNA-590-5p regulates cell viability, apoptosis, migration and invasion of renal cell carcinoma cell lines through targeting ARHGAP24

摘要

Renal cell carcinoma (RCC) is the leading cause of death in renal malignancies. MicroRNA-590-5prn(miR-590-5p) is of great importance in the processes of many cancers regarding regulation of cancerrncell invasion and proliferation. In our study, alternation of miR-590-5p expression in RCC cell linesrnthrough transfection with pre-miR-590-5p (up-regulation) or anti-miR-590-5p (down-regulation) wasrnperformed. Apoptosis and viability of RCC cell lines were measured by flow cytometry and CCK-8rnanalysis, respectively. Cell invasion and migration were estimated by Transwell assay. The association ofrnmiR-590-5p with ARHGAP24 expression was evaluated using luciferase assays, real-time PCR andrnwestern blot assay. The expressions of apoptosis and migration-related protein were also measured byrnwestern blotting. We found that pre-miR-590-5p transfection in Caki-2 and 786-O cells showedrnsignificant increases in cell viability, invasion and migration, which were accompanied by decreased cellrnapoptosis, while anti-miR-590-5p transfection obviously inhibited the cell viability, migration andrninvasion of Caki-2 and 786-O cells as well as induced apoptosis, compared with the negative controlrngroup. Furthermore, bioinformatics combined with luciferase reporter assays indicated that ARHGAP24rnis directly targeted by miR-590-5p. ARHGAP24 overexpression in 786-O and Caki-2 cells phenocopiedrnthe effects of anti-miR-590-5p transfection along with enhanced expression of active Caspase-3 andrnBax/Bcl-2 ratio as well as decreased expression of MMP-2 and MMP-9. These findings suggested thatrnmiR-590-5p/ARHGAP24 seems to function as a potentially beneficial target for RCC treatment.