Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues

摘要

In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC_(50) values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 μg/kg (CLIN) and 6.8 μg/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra -/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC - MS/MS with a correlation coefficient >0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles.