Development of Enzyme-Linked Immunosorbent Assays for Residue Analysis of Diflubenzuron and BAY SIR 8514

摘要

Three enzyme-linked immunosorbent assays (ELISA's) were developed for the benzoylphenylurea insect growth regulators (IGRs) diflubenzuron, BAY SIR 8514, and some of their analogues. All three ELISA's were based on antibodies raised against an N-carboxypropyl hapten of diflubenzuron while a diflubenzuron phenylacetic acid derivative coupled to a different carrier than the immunizing antigen was used as the coating antigen. One ELISA was sensitive for diflubenzuron, BAY SIR 8514, and closely related IGRs while a second assay was sensitive for diflubenzuron while demonstrating minimal cross-reactivity with BAY SIR 8514. In a third case, rabbit anti-diflubenzuron IgG was coupled to alkaline phosphatase, thus eliminating one step in the classical ELISA. None of these assays demonstrated significant cross-reactivity with the benzamide, urea, phenylurea, or aniline components of diflubenzuron or BAY SIR 8514. Each of the three assays was shown to be as sensitive as the recommended HPLC methodology for the analysis of diflubenzuron in water. Low cost was found to be a major advantage of these solid-phase immunoassays over classical procedures. (Copyright (c) 1982, American Chemical Society.)