Selective Labeling of Extracellular Proteins Containing Polyhistidine Sequences by a Fluorescein-Nitrilotriacetic Acid Conjugate

摘要

Fluorescent tags have facilitated the study of protein localization and dynamics involving living cells.The most commonly used labels are fluorescent proteins (FPs).Although of indisputable value,the application of FPs introduces several problems.The size of the FP can prevent a critical component of the labeled construct from achieving its natural structure,function,or locale.Wild-type versions of common FPs have a tendency to oligomerize.Last,the pH sensitivity of FPs limits their ability to image acidic regions of the cell.Genetic modification can sometimes overcome these limitations,but this process is labor-intensive.