Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production and characterization of cellulases

摘要

Solid-state fermentation (SSF) of waste cabbage was carried out by Penicillium notatum NCIM NO-923 for production of extracellular enzymes. Highest activities of carboxymethyl cellulase (CMC) (817.19 ±1.2IU/gds) was obtained at 30℃ and 48 h fermentation time and that of filter paper activity (FPA) (67.49 ±1.33IU/gds) was obtained at 30℃ and 24 h fermentation time. SSF of mixed substrate (cabbage and bagasse; 3:2 w/w) resulted in increase in CMC activity by 3277.3 ±1.22 and FPA by 1280.76 ±1.33. Kinetic parameters were determined from Linewaver Burk plot. Both enzymes exhibited significant thermo stability up to 50℃.