QUANTITATIVE DETERMINATION OF MEMANTINE IN HUMAN PLASMA BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY: APPLICATION TO A BIOEQUIVALENCE STUDY

摘要

A simple, sensitive, and rapid liquid chromatography-tandem mass spectrometry method for determination of memantine in human plasma was established. A one-step protein precipitation with methanol was used to extract the analyte from plasma samples. Memantine and amantadine (internal standard, IS) were separated on a YMC-ODS-C₁₈ column using 0.1% formic acid and methanol as a mobile phase at a flow rate of 0.3 mL/min. Detection was performed on positive ion mode of the transitions at 180.3→107.3 for memantine and 152.2→135.3 for IS by selected reaction monitoring (SRM). The assay was validated over the concentration range of 0.5-50 ng/mL with a lower limit of quantification (LLOQ) of 0.5 ng/mL. The intra- and inter-batch precision (RSD) were no more than 5.96% and 6.37%, respectively. The accuracy was from −3.02% to 7.74%. The validated method was successfully applied to a randomized, 2-period cross-over bioequivalence study in 22 healthy Chinese volunteers following a single oral dose of 10 mg memantine hydrochloride tablet. The simple, inexpensive protein precipitation and high-throughput method makes it a suitable and valuable tool in the investigation of the clinical pharmacokinetics and bioequivalence.View full textDownload full textKeywordsamantadine quantitation, bioequivalence, lC-MS/MS, memantine, pharmacokineticsRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826076.2011.637276