Quantitative analysis of 5-chloro-2-methoxy-n-[2-(4-sulfamoylphenyl)ethyl]benzamide (glyburide analogue, ga) in mouse plasma and whole blood using a micro-extraction and liquid chromatography-tandem mass spectrometry.

摘要

Pharmacokinetic evaluation of 5-chloro-2-methoxy-N-[2-(4- sulfamoylphenyl)ethyl]benzamide in mouse plasma demanded for a suitable bioanalytical method. No reported bioanalytical method exists to-date that can quantify concentration of this compound in any biological matrix. The purpose of this study was 1) to develop and validate a new bioanalytical method using a micro-extraction and LC-MS/MS to quantify the target analyte in mouse plasma and 2) to partially validate the method in whole blood. A bioanalytical method was developed and validated in both matrices for a linear concentration range of 2-1000 ng/ml. For both matrices, the reverse predicted concentration of calibration standards (-8.95% to 12.16% and -9.54% to 12.90% respectively) and precision and accuracy (QCs) were within +/-15% (%RSD and %BIAS). Four-hour bench top stability and post preparative stability results for plasma and whole blood matrices were within +/-15% and +/-20% respectively. Blood --plasma concentration correlation co-efficient was 0.9956 with a slope value of 1.018.