Research Note: Automated Rapid Screening of Foods for the Presence of Salmonellae

摘要

We previously reported an accelerated method to detect salmonellae in foods. It consisted of a preenrichment step (6 h) followed by immunomagnetic separation (IMS) and automated detection, based on positve biochemical characteristics, during overnight incubation. We describe here a simplified procedure with a detection limit of 2 CFU/25 g of food, a procedure that does not require the IMS step. Liquid eggs, milk, ice cream, or poultry were inoculated with Salmonella or non-Salmonella Enterobacteriaceae organisms (2 to 100 CFU/25 g).