Development of a Selective Differential Agar for Isolation and Enumeration of Campylobacter spp.

摘要

Direct plating is an effective technique for isolation and enumeration of Campylobacters from a variety of sample types; however, distinguishing Campylobacters from non-Campylobacter contaminants that frequently grow on many existing agars is difficult. In this study, it was determined that exposing Campylobacter to low levels(200 mg/liter)of riphenyltetrazolium chloride(TTC)was not inhibitory to growth yet was sufficient to give a deep-red to magenta color to the colonies. The new agars(Campy-Line agar[CLA]and Campy-Line blood agar[CLBA])are translucent.