Role of the Glutamate Decarboxylase Acid Resistance System in the Survival of Listeria monocytogenes LO28 in Low pH Foods

摘要

The glutamate decarboxylase (GAD) acid resistance system of the foodborne pathogen Listeria monocytogenes plays a major role in its survival at low pH. It was found that survival of the wild-type strain, LO28, in acidified reconstituted skim milk, diluted to reduce free glutamate levels, improves in response to supplementation with monosodium glutamate. A mutant. In which the two listerial GAD homologs have been deleted (and in which there is no discernible GAD activity), did not Respond to glutamate supplementation and displayed greatly enhanced sensitivity in a number of low pH foods, even when Levels of free glutamate were as low as 0.22 mM.