Fate of Escherichia coli O157:H7 during Composting of Bovine Manure in a Laboratory-Scale Bioreactor

摘要

Inactivation profiles of Escherichia coli O157:H7 in inoculated bovine manure-based compost ingredients were determined by composting these ingredients in a bioreactor under controlled conditions. A 15-liter bioreactor was constructed to determine the fate of E. coli O157:H7 and changes in pH, moisture content, temperature, and aerobic mesophilic and ther-mophilic bacterial counts during composting. Fresh cow manure, wheat straw, cottonseed meal, and ammonium sulfate were combined to obtain a moisture content of ca. 60% and a carbonitrogen ratio of 29:1. The compost ingredients were held in the bioreactor at a constant external temperature of 21 or 50℃. Self-heating of the ingredients due to microbial activity occurred during composting, with stratified temperatures occurring within the bioreactor. At an external temperature of 21℃, self-heating occurred for 0 to 3 days, depending on the location within the bioreactor. E. coli O157:H7 populations increased by 1 to 2 log_(10) CFU/g during the initial 24 h of composting and decreased by ca. 3.5 log_(10) CFU/g near the bottom of the bioreactor and by ca. 2 log_(10) CFU/g near the middle and at the top during 36 days of composting. At an external temperature of 50℃, E. coli O157:H7 was inactivated rapidly (by ca. 4.9 log_(10) CFU/g at the top of the bioreactor, by 4.0 log_(10) CFU/g near the middle, and by 5.9 log_(10) CFU/g near the bottom) within 24 h of composting. When inoculated at an initial level of ca. 10~7 CFU/g, E. coli O157:H7 survived for 7 days but not for 14 days at all three sampling locations, as indicated by either direct plating or enrichment culture. At the top of the bioreactor a relatively constant moisture content of 60% was maintained, whereas the moisture content near the bottom decreased steadily to 37 to 45% over 14 days of composting. The pH of the composting mixture decreased to ca. 6 within 1 to 3 days and subsequently increased to 8 to 9. Results obtained in this study indicate that large populations (10~4 to 10~7 CFU/g) of E. coli O157:H7 survived for 36 days during composting in a bioreactor at an external temperature of 21℃ but were inactivated to undetectable levels after 7 to 14 days when the external temperature of the bioreactor was 50℃. Hence, manure contaminated with large populations (e.g., 10~7 CFU/g) of E. coli O157:H7 should be composted for more than 1 week, and preferably for 2 weeks, when held at a minimum temperature of 50℃.