Optimization of enzymatic hydrolysis of collagen from yellowfin tuna skin (Thunnus albacares) by response surface methodology and properties of hydrolyzed collagen

摘要

Response surface methodology was used to optimize hydrolysis conditions of collagenfrom yellowfin tuna skin (Thunnus albacares) for maximizing the degree ofhydrolysis (DH) and nitrogen recovery efficiency (NR). The results showed that theoptimum hydrolysis parameters were at 54.7°C and pH 8 with 0.034 AU/g alcalaseconcentration for 5.2 hr. The predicted DH and NR under optimal conditionswere validated and confirmed as 24.51% and 96.35%, respectively. The obtainedcollagen hydrolysate (CH) was rich in proline, glycine, methionine, and hydroxyprolineresidues. The antioxidation and antifreezing activity of CH were identified as2,2-diphenyl-1-picrylhydrazylradical activity (IC50 77.04 μg/ml), and hydroxyl radicalactivity (IC50 36.59 μg/ml). It also exhibited antifreezing property and had the inhibitionrate of peroxide value of oil up to 73.53%. The results suggested that CH fromyellowfin tuna skin could be used in the production of functional food products orother related industries.