Cherry stem phenolic compounds: Optimization of extraction conditions and in vitro evaluations of antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and cytotoxic activities

摘要

The present study reported the optimization of extraction of phenolic and flavonoid compounds from the cherry stem and biological activities of the extract. The extraction parameters were optimized employing a response surface methodology, and they were determined as 79°C, 35% (vol/vol) of ethanol percentage and 119 min. It was found that the extract obtained at the optimum conditions contained high amounts of sinapic acid and quercetin followed by caffeic and ferulic acid. It had a high antioxidant capacity and showed antimicrobial activities against Escherichia coli, Enterococcus fecalis, Staphylococcus aureus, Aspergillus flavus, and Aspergillus niger. The extract inhibited α-amylase, α-glucosidase, lipoxygenase, and xanthine oxidase enzymes which indicated that the extract had antidiabetic and anti-inflammatory effects. It also showed cytotoxic activities on the breast and bone cancer cells. The results showed that cherry stems might be potentially useful in food or nonfood applications and an important natural source for bioactive compounds.