The Polymorphisms Of Bovine Cocaine- And Amphetamine-regulated Transcripts And Their Associations With Cattle (bos Taurus) Growth Traits

摘要

We investigated the polymorphisms of bovine cocaine- and amphetamine-regulated transcripts (CART). The coding and regulating regions of CART were screened in 7 cattle breeds by the single-stranded conformation polymorphism (SSCP) technique. The four loci (C1, C2, C3 and C4) studied were all polymorphic. Polymerase chain reaction (PCR) products representing different SSCP variants were sequenced and a total of 9 single-nucleotide polymorphisms (SNPs) were found. The associations between polymorphic loci and the growth traits of Nanyang cattle were analysed. The results indicated that genotype A_1A_1, of the C1 locus was associated with a higher body weight (P < 0.05) than heterozygous A_1B_1. Genotype A_2A_2 of the C2 locus was associated with lower body weight and average daily weight gain (P < 0.001) than heterozygous A_2B_2 C3 and C4 loci had no significant effect on Nanyang cattle growth traits (P > 0.05).