Effect of ±5℃ difference from physiologic temperature on surface area, volume, and surface-to-volume ratio of transformed cells

摘要

Information on the relationship between culture temperature and changes in the cell's area, volume and area to volume (S/V) ratio is lacking. Cancerous epithelial-like cell lines SW480, SW742 and T-47D were cultured at 32, 37 and 42℃ for 48hr. Digital images of cells were analyzed using software Imaged. Observed surface area, volume changes, and surface area to volume (S/V) ratio were significantly different at 32, 37 and 42℃ in the three cell-lines tested (P＜0.05). S/V ratios for all three cell lines at 37℃ were significantly greater than that of at 42℃ (P=0.0001).