Synthesis of conjugated linoleic acid by human-derived Bifidobacterium breve LMC 017: Utilization as a functional starter culture for milk fermentation

摘要

This study was performed to discover bifidobacteria isolated from human intestines that optimally convert linoleic acid (LA) to conjugated linoleic acid (CLA) and to optimize the culture conditions of milk fermentation. One hundred fifty neonatal bifidobacteria were screened for CLA-producing ability, and Bifidobacterium breve LMC 017 was selected as it showed about 90% conversion of free LA in MRS broth. The selected strain showed resistance at 0.5% LA in microaerophilic conditions. When monolinolein (LA 90%) was used as a substrate for CLA production, the con- version rate was lower compared with free LA, but the growth rate was unaffected dur- ing the milk fermentation.