Angiotensin I-converting enzyme inhibitory activity of Acetes chinensis peptic hydrolysate and its antihypertensive effect in spontaneously hypertensive rats

摘要

Peptic hydrolysate with angiotensin I-converting enzyme (ACE) inhibitory activity was prepared from Acetes chinensis. The 3 kDa ultrafiltration filtrates (UF-IV) of the desalted hydrolysate exerted a potent ACE inhibitory activity with IC50 being 0.67 mg mL~(-1). The fractions with molecular weight located between 611 and 240 Da achieved a total percentage of 63.6% in the UF-IV, with a mean peptide length of about 5.6-2.1. Gastrointestinal digestions were simulated on the hydrolysate and UF-IV and addressed their effect on the ACE inhibitory activity. The ACE inhibitory activity of the UF-IV could survive or be maintained in active form by simulated gastrointestinal digestion. Furthermore, the UF-IV exerted potent antihypertensive effect after oral administrations were given to spontaneously hypertensive rats (SHR) at a dose of 900 mg kg~(-1) body weight. In conclusion, the UF-IV could serve as useful antihypertensive peptides in the prevention and treatment of hypertension and other associated disorders.