Myoglobin: A promising exogenous reference marker using in proteomics analysis

摘要

In 2-dimensional gel electrophoresis (2-DE) analysis, quantification of protein expression difference is difficult because of the high variation of spot sizes among gels. Therefore, identification of a reference marker for data normalization is necessary. In this study, consistent spot size and high reproducibility of myoglobin in 2-DE gels were successfully achieved. The use of myoglobin not only improved quantification of protein expression differences in images from different samples of the same tissue, but also made the comparison of difference in protein expression among different tissues possible. Thus, myoglobin is promising as a reference marker for data normalization in proteomic analysis.