Establishment of a stable Amaranthus tricolor callus line for production of food colorant

摘要

The present study attempts to establish and screen Amaranthus tricolor L. callus for development of a stable callus line with enhanced production of food colorants. The best betacyanin-producing callus was induced on Murashige and Skoog basal medium supplemented with 1-naphthalene acetic acid (NAA) (0.25 mg/L) and 6-benzylaminopurine (BA) (2 mg/L) using stem segment as explants. The callus line (IIT BT/PBT At 11) was maintained up to 24 subculture cycles. Betalain production in callus was detected by reversed phase HPLC and LCMS. In addition to the reported abundant red-violet pigment amaranthin, 2 new yellow pigments and 18 other bioactive phenylpropanoids were detected in the callus line. A novel betaxanthin, methyl derivative of arginine betaxanthin was identified. All pigments were purified by size exclusion chromatography.