大豆加工食品を対象とした遺伝子絤搎え食品検査におけるdna抽出方法の検討

摘要

In order to evaluate three different methods for DNA extraction (CTAB, DNeasy Plant Mini Kit and Wizard DNA Clean-up Resin system), the yields of DNA extracted from soyproducts and the copy numbers of lectin genes amplified by quantitative PCR were compared. Fermented foods, such as miso and nattou, gave poor yields of DNA and low copy numbers with any method. However atsu-age and kinugoshi-tofu gave high-quality results with all methods. Kinako gave a high yield of DNA, but poor amplification. Boiled soybeans and soymilk showed in poor amplification. It is important to choose the appropriate DNA extraction method for each product.%遺伝子組換え食品の試験法は，安全性審査の義務化と表rn示制度を監視するため開発され，厚生労働省通知「組rn換えDNA技術応用食品の検査法について」（以下，厚rn労省通知法と略称する）および農林水産消費技術センターrnが作成したJAS分析試験ハンドブック「遺伝子組換え食rn品検査・分析マニュアル」（以下，JAS試験法と略称すrnる）に示されている．