Byssochlamys spp.同定のための遺伝子指標の評価

摘要

Comparative sequence analysis was performed on the 18S rRNA gene (1,676 bp), 26/28S rRNA gene D2 region (321 bp) and lys2 (997 bp) to evaluate the gene index for rapid, accurate and convenient identification of Byssochlamys spp. and related species. The results showed that 26 strains (11 species) of the clade could be identified or grouped by means of each gene sequence. The highest resolution to discriminate these species was observed with lys2, but 26/28S rRNA gene D2 region was considered to be the best index for convenient identification. In addition, phylogenetic analysis based on these genes indicated that genus Byssochlamys is not mono-phyletic, although species in the clade are closely related to each other. Re-classification will be necessary, based on detailed morphological observations.%耐熱性カビは，子嚢菌門に属し，75℃・30分の加熱（湿熱）にも生残する菌として知られている．完全世代（テレオモルフ）の菌としてはByssochlamys属，Eupenicillium属，Neosartorya属およびTalaromyces属が代表的で，はかにEurotium属，Hamigera属およびThermoascus属が知られており，これらの子嚢胞子や厚膜胞子が高い耐熱性を示す．