Response surface methodology for extraction optimization of pigeon pea protein

摘要

Optimisation for pigeon pea protein extraction (Y) (Cajanus cajan (L) Mi1lsp from the new IAPAR 43-Arata variety) was investigated using response surface methodology. A compound central design was used with variables (X_l) NaCl concentration (0.000; 0.025, 0.050, 0.075 and 0.100M), (X_2) pH (2.5; 4.0; 5.5; 7.0 and 8.5) and (X_3) liquidosolid ratio (5:l, l0:l, l5:1; 20f:l; and 25:l, v/w). A model of the second degree equation was used to create the surface responses and confirmative studies were carried out. The following equation: Y=-l9.3733 + 8.6004x_2-0.508526x~2_2 shows optimum conditions for protein extraction of about 75/100 yield, at pH 8.5 without NaCl regardless of the liquidfso1id ratio (v/w) under the experimenta1 conditions studied.