Biochemical and immunochemical detection of types Ⅰ and Ⅴ collagens in tiger puffer Takifugu rubripes

摘要

Crude collagen fractions, prepared by alkali extraction from several parts (muscle, liver, swim bladder, bone, fin, gill, alimentary tract) of tiger puffer Takifugu rubripes, were analysed by biochemical and immunochemical techniques to examine the distribution of collagen types (types Ⅰ and Ⅴ collagens) among these parts. Collagens from these parts showed quite similar SDS-PAGE patterns and similar peptide maps. Immunoblot analyses were performed for these collagens using antisera against types Ⅰ and Ⅴ collagens isolated from pepsin-solubilized collagen of skin, revealing the existence of the positive components for these antisera in all of them. The results suggest that types Ⅰ and Ⅴ collagens may be widely distributed in these parts of tiger puffer.