A convenience UPLC/PDA method for the quantitative analysis of panaxfuraynes A and B from Panax ginseng

摘要

Panaxfurayne A and B, biologically novel tetrahydrofuranic polyacetylene glycosides, were founded from roots of Panax ginseng C. A. Meyer (Araliaceae). To study the contents of panaxfurynes A and B, a quantitative analysis method was developed using ultra performance liquid chromatography (UPLC) coupled with photo diode array detector. The dried (65 ℃, 72 h) and powdered sample (5 g) was extracted with ethanol (15%, 100 ml) for 30 min at 50 ℃ and concentrate. The constituents were separated using an iso-cratic mobile phase consisting acetonitrile (16%) in water for 10 min on a ODS column. The contents of panaxfuraynes A and B in the roots of Panax quinquefolium, Panax japonicum, Panax notoginseng and P. ginseng were found to less than 3 and 2 ng/g approximately, respectively. The constituents were not detected from P. japonicum.