首页> 外文期刊>Food Chemistry >In-depth glycoproteomic characterisation of grape berry vacuolar invertase using a combination of mass spectrometry-based approaches
【24h】

In-depth glycoproteomic characterisation of grape berry vacuolar invertase using a combination of mass spectrometry-based approaches

机译:结合基于质谱的方法对葡萄浆果液泡型转化酶进行深入的蛋白质组学表征

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

Vacuolar invertase is a key enzyme of sugar metabolism in grape berries. A full characterisation of this highly N-glycosylated protein is required to help understand its biological and biochemical significance in grapes. We have developed a mass spectrometry (MS)-based glycoproteomic approach wherein deglycosylated peptides are analysed by LC-MS/MS, while intact glycopeptides are characterised using a dedicated MS method to determine the attachment sites and micro-heterogeneity. For grape invertase, in parallel with deglycosylated peptides analysis, different enzymatic digestions were performed and glycopeptide detection was improved by enrichment method, nanoLC-MS and oxonium glycan ions. This MS-based glycoproteomic approach demonstrates that vacuolar invertase is glycosylated at all twelve potential N-glycosylation sites. Glycosylation is heterogeneous, with twelve glycoforms identified at six of the sites. The identification of several types of N-glycans is a major result to correlate with the surface and foaming properties of wine, the solubility, allergenicity, and protease resistance of wine proteins. (C) 2016 Elsevier Ltd. All rights reserved.
机译:泡状转化酶是葡萄浆果糖代谢的关键酶。需要充分表征这种高度N-糖基化的蛋白质,以帮助了解其在葡萄中的生物学和生化意义。我们已经开发了一种基于质谱(MS)的糖蛋白组学方法,其中通过LC-MS / MS分析去糖基化的肽,而完整的糖肽则使用专用的MS方法进行表征,以确定连接位点和微异质性。对于葡萄转化酶,与去糖基化肽段分析同时进行不同的酶消化,并通过富集法,nanoLC-MS和氧代糖基聚糖离子改善糖肽的检测。这种基于质谱的糖蛋白组学方法表明液泡转化酶在所有十二个潜在的N-糖基化位点均被糖基化。糖基化是异质的,在六个位点鉴定出十二种糖型。鉴定几种类型的N-聚糖是与葡萄酒的表面和起泡特性,葡萄酒蛋白的溶解度,变应原性和蛋白酶抗性相关的主要结果。 (C)2016 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Food Chemistry》 |2016年第1期|237-244|共8页
  • 作者单位

    Univ Strasbourg, IPHC, BioOrgan Mass Spectrometry Lab LSMBO, 25 Rue Becquerel, F-67087 Strasbourg, France|CNRS, IPHC, UMR7178, F-67087 Strasbourg, France;

    Univ Strasbourg, IPHC, BioOrgan Mass Spectrometry Lab LSMBO, 25 Rue Becquerel, F-67087 Strasbourg, France|CNRS, IPHC, UMR7178, F-67087 Strasbourg, France;

    Univ Strasbourg, IPHC, BioOrgan Mass Spectrometry Lab LSMBO, 25 Rue Becquerel, F-67087 Strasbourg, France|CNRS, IPHC, UMR7178, F-67087 Strasbourg, France;

    Univ Reims, URVVC EA 4707, Lab Oenol & Chim Appl, Moulin Housse,BP 1039, F-51687 Reims 2, France;

    Univ Reims, URVVC EA 4707, Lab Oenol & Chim Appl, Moulin Housse,BP 1039, F-51687 Reims 2, France;

    Univ Strasbourg, IPHC, BioOrgan Mass Spectrometry Lab LSMBO, 25 Rue Becquerel, F-67087 Strasbourg, France|CNRS, IPHC, UMR7178, F-67087 Strasbourg, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Vacuolar invertase; Grape; N-Glycosylation; Mass spectrometry; Glycopeptide; Deglycosylation; Wine;

    机译:液泡蔗糖酶;葡萄;N-糖基化;质谱;糖肽;去糖基化;酒;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号