Rapid visual sensing and quantitative identification of duck meat in adulterated beef with a lateral flow strip platform

摘要

A novel high-sensitivity authentication method has been demonstrated for the rapid visual detection of adulterated meat based on both the lateral flow strip (LFS) platform and on polymerase chain reaction (PCR). After the rapid extraction of genomic components from meat, the on-site amplification of the target DNA of adulterated duck meat is carried out with the rationally designed functional FITC- and biotin-modified primer set, thereby producing numerous double-stranded DNA (dsDNA) products dually labelled with FITC and biotin. The FITC-labelled terminal end of the products binds to the pre-immobilized FITC antibody on the test line of the strip, and the biotin-labelled terminal end binds to the streptavidin-conjugated gold nanoparticles, resulting in a visible WA line on the LFS for the rapid identification of duck meat in adulterated beef. After optimization, an adulteration ratio as low as 0.05% can be easily measured, which is more sensitive than other common adulteration authentication methods and is even comparable to instrumental methods. Moreover, 22 commercial processed meat samples were tested with this new strategy, and 4 adulterated samples were successfully identified by both the classic method and our method. In essence, the present authentication method is simple in design, convenient in operation, and can be easily extended to the identification of other adulteration components just by replacing the modified primers.