Potentiation of the effect of a commercial animal feed additive mixed with different probiotic yeast strains on the adsorption of aflatoxin B_1

摘要

This study potentiates the adsorbent effect for aflatoxin B_1 (AFB_1) of a commercial additive (CA) of animal feed, containing inactive lysate of three Saccharomyces cerevisiae strains, active enzymes, adsorbents and a selenium-amino acid complex, when the additive was mixed separately with three 5. cerevisiae strains. Levels of AFB_1 of 20 and 50 ng g~(-1) were used to determine the binding capacity of different concentrations of CA alone and in the presence of yeast strains, as well as toxin desorption, under gastrointestinal conditions. The viability of yeasts in the presence of CA was evaluated. The results show that the CA did not affect the viability of the yeast strains assayed. CA alone showed a low percentage adsorption. At 20 and at 50 ng g~(-1), CA was highly efficient in adsorbing AFB_1 when combined with RC016 and RC012 strains respectively. Desorption of AFB_1 by CA alone and in combination with the yeasts increased with increasing levels of CA. The results demonstrate the improvement of CA in AFB_1 adsorption once it is mixed with live yeasts.