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Limited but specific variations of seed storage proteins in Japanese common wheat (Triticum aestivum L.)

机译:日本普通小麦(Triticum aestivum L.)的种子贮藏蛋白有限但特定的变异

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摘要

The electrophoretic banding patterns ofgliadin in common wheat lines derived fromJapan were determined byacid-polyacrylamide gel electrophoresis. For the 107 wheat lines used in our study,27 different patterns were identified, 13corresponding to the ο-gliadin, 8 tothe β, γ-gliadin and 6 to theα-gliadin. The gliadin patterns ofJapanese wheat cultivars and landracesgreatly differed from the patterns of wheatlines from other countries, and thevariation seen in wheat lines from Japanwas limited to 46 patterns. Sevencollection or breeding areas in Japanshowed different frequencies in theirgliadin patterns. Combining the gliadinpatterns with high molecular weightglutenin subunit compositions, 67combinations were observed. One gliadinpattern consisting of ο-gliadinpattern F, β, γ-gliadinpattern H and α-gliadin pattern Dwas frequently found in many Japanese wheatlines, though the other patterns werelimited to only one or two wheat lines.
机译:通过酸-聚丙烯酰胺凝胶电泳确定了日本普通小麦品系中麦醇溶蛋白的电泳谱带图谱。在本研究中使用的107个小麦品系中,共鉴定出27种不同模式,其中13种对应于麦醇溶蛋白,o-麦醇溶蛋白对应8种,β,γ-麦醇溶蛋白对应6种,α-麦醇溶蛋白对应。日本小麦品种和地方品种的麦醇溶蛋白模式与其他国家的小麦系模式大不相同,日本小麦系的差异仅限于46种模式。日本的七个采集区或繁殖区的胶质模式显示出不同的频率。将麦醇溶蛋白模式与高分子量谷蛋白亚基组合物组合,观察到67种组合。在许多日本小麦品系中经常发现一种麦醇溶蛋白模式,由麦醇溶蛋白模式F,β,γ-麦醇溶蛋白模式H和α-麦醇溶蛋白模式D组成,尽管其他模式仅限于一两个小麦谱系。

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