The role of TGF-β1/Smad3 signaling pathway and oxidative stress in the inhibition of osteoblast mineralization by copper chloride

摘要

To explore the relationship of oxidative stress and TGF-β1/Smad3 pathway in the inhibition of osteoblast mineralization by copper chloride (CuCl_2), the osteoblasts were treated with CuCl_2 (0, 50 μM, 100 μM, 150 μM CuCl_2 5H_2O) for 24 h. We found that Cu impaired the osteoblast structure, inhibited the glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, alkaline phosphatase (ALP) content, mRNA expression of collagen Ⅰ (COL-Ⅰ), osteocalcin (OCN), insulin-like growth factor Ⅰ (IGF-Ⅰ), bone morphogenetic protein-2 (BMP-2), transforming growth factor β1 (TGF-β1) and core-binding factor α1 (Cbfα1), promoted the reactive oxygen species (ROS) production, inactivated the TGF-β1/Smad3 pathway. It indicates that the inactivated TGF-β1/ Smad3 pathway leads to osteoblast impairment by CuCl_2. It will contribute to clarify the influence of CUCl_2 on the osteoblast mineralization.