Prologed Oxidative Stress Impairs Insulin-Induced GLUT4 Translocationin 3T3-L1 Adipocytes

摘要

Prolonged exposure of 3T3-L1 adipocytes to micromo- lar concentrations of H_2O_2 results in an impaired response to the acutemetabolic effects of insulin. In this study, we further characterized the mechanisms by which oxidative stress impairs insulin stimulation of glucose transport activity. Although insulin induced a 2.5-fold increase in plasma membrane (GLUT4 content and a 50/100 reduction in its abundance in the low-density microsomal (LDM) fraction in control cells, oxidation completely prevented these responses.