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RNA-seq data from whole rice grains of pigmented and non-pigmented Malaysian rice varieties

机译:来自针颜料和非着色的马来西亚水稻品种的全水稻颗粒的RNA-SEQ数据

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Pigmented rice is enriched with antioxidants, macro- and micronutrients. A comprehensive investigation of the gene expression patterns among the pigmented rice varieties would help to understand the cellular mechanism and biological processes of rice grain pigmentation. Hence, we performed RNA sequencing and analysis on the whole grain of dehusked mature seeds of selected six Malaysian rice varieties with varying grain pigmentations. These varieties were black rice (BALI and Pulut Hitam 9), red rice (MRM16 and MRQ100) and white rice (MR297 and MRQ76). Illumina HiSeq? 4000 sequencer was used to generate total raw nucleotides of approximately 53 Gb in size. From 353,937,212 total paired-end raw reads, 340,131,496 total clean reads were obtained. The raw reads were deposited into European Nucleotide Archive (ENA) database and can be accessed via accession number PRJEB34340. This dataset allows us to identify and profile all expressed genes with functions related to nutritional traits (i.e. antioxidants, folate and amylose content) and quality trait (i.e. aroma) across both pigmented and non-pigmented rice varieties. In addition, the transcriptome data obtained will be valuable for discovery of potential gene markers and functional SNPs related to functional traits to assist in rice breeding programme.
机译:用抗氧化剂,宏观和微量营养素富集着色米饭。对着色水稻品种中基因表达模式进行全面调查,有助于了解水稻籽粒色素沉着的细胞机制和生物过程。因此,我们对选择六个马来西亚水稻品种的全粒粮食的RNA测序和分析,不同的粒子色素沉着。这些品种是黑米(巴厘岛和普拉姆9),红米(MRM16和MRQ100)和白米(MR297和MRQ76)。 Illumina Hiseq? 4000序列子用于产生大约53gB的总原始核苷酸。从353,937,212总配对的原始读数,获得了340,131,496的总清洁读数。原始读数沉积在欧洲核苷酸存档(ENA)数据库中,可以通过加入号PRJEB34340访问。该数据集允许我们识别和配置所有表达的基因,其功能与营养性状相关的功能(即抗氧化剂,叶酸含量)和质量性状(即香气)横跨着色和非着色水稻品种。此外,所获得的转录组数据对于发现与功能性性状有关的潜在基因标记和功能性SNP具有有价值,以协助水稻育种计划。

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