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RNA-seq data from whole rice grains of pigmented and non-pigmented Malaysian rice varieties

机译:来自有色和无色马来西亚大米品种全谷粒的RNA-seq数据

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摘要

Pigmented rice is enriched with antioxidants, macro- and micronutrients. A comprehensive investigation of the gene expression patterns among the pigmented rice varieties would help to understand the cellular mechanism and biological processes of rice grain pigmentation. Hence, we performed RNA sequencing and analysis on the whole grain of dehusked mature seeds of selected six Malaysian rice varieties with varying grain pigmentations. These varieties were black rice (BALI and Pulut Hitam 9), red rice (MRM16 and MRQ100) and white rice (MR297 and MRQ76). Illumina HiSeq™ 4000 sequencer was used to generate total raw nucleotides of approximately 53 Gb in size. From 353,937,212 total paired-end raw reads, 340,131,496 total clean reads were obtained. The raw reads were deposited into European Nucleotide Archive (ENA) database and can be accessed via accession number PRJEB34340. This dataset allows us to identify and profile all expressed genes with functions related to nutritional traits (i.e. antioxidants, folate and amylose content) and quality trait (i.e. aroma) across both pigmented and non-pigmented rice varieties. In addition, the transcriptome data obtained will be valuable for discovery of potential gene markers and functional SNPs related to functional traits to assist in rice breeding programme.
机译:色素大米富含抗氧化剂,大量和微量营养素。对有色水稻品种之间基因表达模式的全面研究将有助于了解水稻籽粒色素沉着的细胞机制和生物学过程。因此,我们对选定的六种具有不同谷物色素的马来西亚水稻品种的去壳成熟种子的全谷物进行了RNA测序和分析。这些品种是黑米(BALI和Pulut Hitam 9),红米(MRM16和MRQ100)和白米(MR297和MRQ76)。 Illumina HiSeq™4000测序仪用于生成大小约为53 Gb的总原始核苷酸。从353,937,212个配对末端原始读取中,总共获得了340,131,496个干净读取。原始读物已存入欧洲核苷酸档案库(ENA)数据库,可通过登录号PRJEB34340进行访问。该数据集使我们能够识别和分析所有有色和无色水稻品种中与营养性状(即抗氧化剂,叶酸和直链淀粉含量)和质量性状(即香气)有关的所有表达基因。另外,获得的转录组数据对于发现潜在的基因标记和与功能性状有关的功能性SNPs具有重要价值,以协助水稻育种计划。

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