Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp

摘要

Phytoeno desaturase (pds gene) is an enzyme involved in the biosynthesis of carotenoids such as β-carotene and astaxanthin in microalgae and some autotrophic organisms. Carotenoids have antioxidant, anti-inflammatory and anticarcinogenic properties. Prior to biotechnological studies it is necessary to carry out the integral analysis of this carotenogenic gene and its molecules for future industrial applications. In silico modeling is an important bioinformatics tool that allows to adjust, summarize and organize experimental information, test theories and generally understand the interaction of molecules and biological systems. In this work, specific primers were used to amplify the phytoene desaturase (pds) gene of Haematococcus sp. (PM015). NEBcutter V2.0 and BioEdit provided the simulation design of the pds restriction patterns together with the restriction enzymes FsPI, AlwI, HincII, BlpI and MluCI. The amplification product resulted in two fragments one of 1200 bp and one of 1700 bp. The in silico design was reproduced and tested experimentally, in this way the identification of the pds gene of Haematococcus sp. (PM015) and the effectiveness of bioinformatics programs as an important alternative for in silico modeling of molecules and/or biological systems, which mapping predict reliable results, saving time and reducing costs in the experimental stage, in addition to allowing the selection of restriction enzymes with optimal enzymatic activity to digest DNA.