Synthesis and Crystal Structure Studies of Mitomycin C Dihydrate

摘要

The absolute configuration of mitomycin C was determined? by X-ray single crystal diffraction ?(Cu K α ), and a new? crystalline ?dihydrate of mitomycin C had been prepared. The? experiment ?result? provide s ?a definitive answer ?to the real absolute configuration of mitomycin C and may put an end to the dispute? that baffle s ?researchers for decades .? A t the same time, some contentious structures ? about the mitomycin C in? American Pharmaco p oeia ? USP 36 -NF 31 ,? Chinese pharmacopoeia ? 2015 ?edtion ?and numbers of? literature s are marked. The absolute configuration? is? also verified ?by ? 1D ( ~( 1 ) H ?and? ~( 13 ) C) and 2D (HSQC, HMBC,? ~( 1 ) H- ~( 1 ) H? COSY ?and? NOESY) NMR studies ?indirectly. P owder X - ray diffraction ( P XRD) pattern of the? mitomycin C dihydrate ?is similar to that calculated for? it, which? suggests that the purity? of? sample? is excellent .