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首页> 外文期刊>Journal of Clinical Microbiology >Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of Escherichia coli
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Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of Escherichia coli

机译:快速检测大肠杆菌常见菌株的环介导等温扩增测定

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摘要

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.
机译:我们开发了针对大肠杆菌的高灵敏度和特异性LAMP检测方法。它不需要DNA提取,最多可检测10个拷贝。它检测到36个 E中的所有36个。大肠埃希菌分离物和所有22种尿液样本(测试的89个样本中)都含有 E。大肠杆菌。该测定快速,成本低并且易于执行。

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