Establishment of Immunological-Based Assay and Molecular Assay for Rapid Detection of Vibrio harveyi

摘要

Monoclonal antibodies (MAbs) can be divided2 groupsby dot blotting. The first group consisted of MAb VH9-11B and VH20-9C specific to 36 and 27 isolates ofV. harveyi, respectively and showed cross reactivity withV. campbellii3 isolates. The second group, of MAb VH15-6G and VH17-1G were specific to certain isolates ofV. harveyiderived from different sources. In case of the PCR and loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD) methods targeting thevhhP2gene ofV. harveyi, both methods accurately identified 22 isolates ofV. harveyibut did not detect 16 non-harveyiVibrioisolates, and 34 non-Vibrio bacterial isolates that can be used to distinguishV. harveyifromV. campbellii.