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Activation of bile salt dependent lipase by (lyso)phosphatidic acid and platelet activating factor

机译:(溶血)磷脂酸和血小板活化因子对胆汁盐依赖性脂肪酶的活化

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摘要

TheactivityofbreastmilkBSDLwasassayedwithorwithoutphospholipidsasextra‐intestinaleffectorcandidates.Phosphatidicacid,lysophosphatidicacidandplateletactivatingfactorbutnotphosphatidylcholineandlysophosphatidylcholinestimulatedBSDLactivityatleastasefficientlyastaurocholate.TheapparentdissociationconstantsofPAandLPAatsaturatingconcentrationsofthreedifferentsubstrateswerebetween0.1and13.4μMandthatofPAFwasbeloworequalto200pM.Kineticdatasuggestedtheexistenceofatleastonebindingsiteforeachoftheseeffectors.PA,LPAandPAFarelikelyextra‐intestinalmodulatorsofBSDLactivity...
机译:TheactivityofbreastmilkBSDLwasassayedwithorwithoutphospholipidsasextra-intestinaleffectorcandidates.Phosphatidicacid,lysophosphatidicacidandplateletactivatingfactorbutnotphosphatidylcholineandlysophosphatidylcholinestimulatedBSDLactivityatleastasefficientlyastaurocholate.TheapparentdissociationconstantsofPAandLPAatsaturatingconcentrationsofthreedifferentsubstrateswerebetween0.1and13.4μMandthatofPAFwasbeloworequalto200pM.Kineticdatasuggestedtheexistenceofatleastonebindingsiteforeachoftheseeffectors.PA,LPAandPAFarelikelyextra-intestinalmodulatorsofBSDLactivity ...

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    《FEBS Letters》 |2013年第18期|共6页
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  • 中图分类 分子生物学;
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