Cloning and characterization of the cDNA encoding rice elongation factor 1β

摘要

>We have cloned and sequenced a cDNA coding for rice elongation factor 1β (EF-1β). The clone was 1420 bp long and contained an open reading frame coding for 229 amino acids. The overall identity between rice EF-1β and rice EF-1β' [Matsumoto, S., Oizumi, N., Taira, H. and Ejiri, S. (1992) FEBS Lett. 311, 46-48] is 60% at the amino acid sequence level; a higher percent identical residues (81%) were especially observed in the C-temiinal region. Rice EF-1β has no conserved phosphorylation site for casein kinase II and no leucine zipper motif, although these motifs are well conserved in EF-1δ (= β in plants) subunits of animal EF-1.