Isolation and characterization of a cDNA clone encoding one IgE-binding fragment of Penicillium brevicompactum

摘要

Pen b 26 was isolated as an allergen of Penicillium brevicompactum, which is known to be one of the major source of indoor fungal allergies. Pen b 26 was isolated and cloned into Escherichia coli as an N-terminus his-tagged fusion protein, which had a calculated molecular weight of 14.9 kDa while the native Pen b 26 was 11 kDa. The over-expressed fusion protein migrated at about 20 kDa on SDS-PAGE although its analysis by mass spectroscopy confirmed its calculated size. The IgE antibodies in the sera of 25% Penicillium-allergic individuals showed positive reaction to the purified fusion protein. Pen b 26 was also identified as a 60S ribosomal P1 phospho-protein. This invention includes a cDNA sequence from which the recombinant allergen was produced.